Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy.
Identifieur interne : 000B35 ( Main/Exploration ); précédent : 000B34; suivant : 000B36Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy.
Auteurs : Andreas J. Meyer [Allemagne] ; Thorsten BrachSource :
- Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2009.
Descripteurs français
- KwdFr :
- Arabidopsis (génétique), Arabidopsis (métabolisme), Cinétique (MeSH), Disulfures (métabolisme), Glutarédoxines (métabolisme), Glutathion (métabolisme), Microscopie confocale (méthodes), Oxydoréduction (MeSH), Plantes (génétique), Plantes (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Stress oxydatif (MeSH).
- MESH :
- génétique : Arabidopsis, Plantes, Protéines à fluorescence verte.
- métabolisme : Arabidopsis, Disulfures, Glutarédoxines, Glutathion, Plantes, Protéines à fluorescence verte.
- méthodes : Microscopie confocale.
- Cinétique, Oxydoréduction, Stress oxydatif.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Disulfides (metabolism), Glutaredoxins (metabolism), Glutathione (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Kinetics (MeSH), Microscopy, Confocal (methods), Oxidation-Reduction (MeSH), Oxidative Stress (MeSH), Plants (genetics), Plants (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins.
- chemical , metabolism : Disulfides, Glutaredoxins, Glutathione, Green Fluorescent Proteins.
- genetics : Arabidopsis, Plants.
- metabolism : Arabidopsis, Plants.
- methods : Microscopy, Confocal.
- Kinetics, Oxidation-Reduction, Oxidative Stress.
Abstract
Continuous control of metabolism and development is a key feature of life and is of particular importance under stress conditions. While under normal conditions most cellular compartments maintain a reducing environment, the cellular redox state can be influenced by external factors. Redox changes might in turn be employed as part of a signalling cascade leading to molecular responses to adverse situations. To enable dynamic measurements of the cellular redox poise in vivo, reduction-oxidation sensitive GFP (roGFP) can be expressed in plant cells and observed by confocal microscopy. When imaged by confocal microscopy this probe exhibits significant opposing shifts in the fluorescence intensities excited at 488 and 405 nm upon formation of an intramolecular disulfide bridge, which enables ratiometric analysis. The formation of the disulfide bridge is directly responsive to the redox state of the glutathione redox buffer within the subcellular compartment to which roGFP is targeted.
DOI: 10.1007/978-1-59745-289-2_6
PubMed: 19083173
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Meyer</name><affiliation wicri:level="3"><nlm:affiliation>Heidelberg Institute of Plant Sciences, University of Heidelberg, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Heidelberg Institute of Plant Sciences, University of Heidelberg, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Brach, Thorsten" sort="Brach, Thorsten" uniqKey="Brach T" first="Thorsten" last="Brach">Thorsten Brach</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19083173</idno><idno type="pmid">19083173</idno><idno type="doi">10.1007/978-1-59745-289-2_6</idno><idno type="wicri:Area/Main/Corpus">000B41</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B41</idno><idno type="wicri:Area/Main/Curation">000B41</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B41</idno><idno type="wicri:Area/Main/Exploration">000B41</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy.</title><author><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. 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While under normal conditions most cellular compartments maintain a reducing environment, the cellular redox state can be influenced by external factors. Redox changes might in turn be employed as part of a signalling cascade leading to molecular responses to adverse situations. To enable dynamic measurements of the cellular redox poise in vivo, reduction-oxidation sensitive GFP (roGFP) can be expressed in plant cells and observed by confocal microscopy. When imaged by confocal microscopy this probe exhibits significant opposing shifts in the fluorescence intensities excited at 488 and 405 nm upon formation of an intramolecular disulfide bridge, which enables ratiometric analysis. The formation of the disulfide bridge is directly responsive to the redox state of the glutathione redox buffer within the subcellular compartment to which roGFP is targeted.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19083173</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN><JournalIssue CitedMedium="Print"><Volume>479</Volume><PubDate><Year>2009</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy.</ArticleTitle><Pagination><MedlinePgn>93-107</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-59745-289-2_6</ELocationID><Abstract><AbstractText>Continuous control of metabolism and development is a key feature of life and is of particular importance under stress conditions. While under normal conditions most cellular compartments maintain a reducing environment, the cellular redox state can be influenced by external factors. Redox changes might in turn be employed as part of a signalling cascade leading to molecular responses to adverse situations. To enable dynamic measurements of the cellular redox poise in vivo, reduction-oxidation sensitive GFP (roGFP) can be expressed in plant cells and observed by confocal microscopy. When imaged by confocal microscopy this probe exhibits significant opposing shifts in the fluorescence intensities excited at 488 and 405 nm upon formation of an intramolecular disulfide bridge, which enables ratiometric analysis. The formation of the disulfide bridge is directly responsive to the redox state of the glutathione redox buffer within the subcellular compartment to which roGFP is targeted.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>Andreas J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Heidelberg Institute of Plant Sciences, University of Heidelberg, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brach</LastName><ForeName>Thorsten</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004220">Disulfides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004220" MajorTopicYN="N">Disulfides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>12</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>12</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19083173</ArticleId><ArticleId IdType="doi">10.1007/978-1-59745-289-2_6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li></region><settlement><li>Heidelberg</li></settlement></list><tree><noCountry><name sortKey="Brach, Thorsten" sort="Brach, Thorsten" uniqKey="Brach T" first="Thorsten" last="Brach">Thorsten Brach</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. 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